Note: When tested with the Limulus Color KY series, the potency is shown on the CSE vial label. Divide the potency (EU/vial) by 1,000 to calculate the volume of LRW needed to reconstitute CSE for a yield of 1,000 EU/mL.

Example: Labeled Sensitivity (λ) of PYROSTAR™ ES-F Multi-Test Reagent: 0.015 EU/mL

The final endotoxin (dilution tubes 8, 9, 10 and 11) would be achieved to generate a standard of dilutions to assess labeled sensitivity of 0.015 EU/mL.

Note: This is an example of how to perform the double dilution. It is acceptable to adjust the volumes of LRW and endotoxin if the ratios are maintained.